Author Question: Explain how a sample brain is processed during the histology process. What will be an ideal ... (Read 34 times)

dmcintosh · **on:** Jun 22, 2018

Explain how a sample brain is processed during the histology process.

What will be an ideal response?

Question 2

Give an overview of stereotaxic surgery for the purpose of implanting a cannula into the brain.

What will be an ideal response?

billybob123 · **Reply #1 on:** Jun 22, 2018

Answer to Question 1

Answer: The brain is perfused to remove blood from the tissue and is fixed in formalin to harden the tissue and to halt autolysis. Stains are applied to the section to reveal the internal details of the brain section.

Answer to Question 2

Answer: The target of the cannula is decided upon and located in a stereotaxic atlas. The coordinates for placement involve distances in mm from bregma (skull landmark), the midline, and beneath the surface of the skull. A stereotaxic instrument is used to hold the head of an anesthetized rat solidly in place. After incising and retracting the scalp, a hole is drilled through the skull and the cannula inserted into the brain, just above the target site. Dental acrylic and small metal screws are used to hold the cannula in place.