This topic contains a solution. Click here to go to the answer

Author Question: Explain how a particular neurotransmitter receptor is localized in brain. What will be an ideal ... (Read 15 times)

penguins

  • Hero Member
  • *****
  • Posts: 903
Explain how a particular neurotransmitter receptor is localized in brain.
 
  What will be an ideal response?

Question 2

Contrast efferent and afferent tracing procedures.
 
  What will be an ideal response?



Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by a Subject Expert

Hikerman221

  • Sr. Member
  • ****
  • Posts: 346
Answer to Question 1

Answer: One way is to use autoradiography in which brain tissue is exposed to a solution of a radioactive receptor antagonist. In the second method, antibodies are proteins produced by white blood cells that are specific to another protein or peptide (antigen). Receptors are comprised of proteins (antigens), for which an antibody can be developed and tagged using a marker such as a fluorescent marker (or dye). A slice of brain tissue is incubated with the antibody/marker so that the antibody will attach to the antigen (receptor) with each location indicated by the presence of a fluorescent signal.

Answer to Question 2

Answer: In the efferent tracing procedure, a chemical such as PLA is injected into a brain site, taken up by dendrites and transported out to the axons. Immunocytochemistry is used to visualize the places that receive axons from the neurons of the injection site. In contrast, afferent tracing involves injecting a chemical, such as fluorogold, which is taken up by axon terminal buttons and transported backwards to the cell bodies. This procedure thus identifies the neurons that innervate a specific brain region.




penguins

  • Member
  • Posts: 903
Reply 2 on: Jun 22, 2018
Gracias!


bdobbins

  • Member
  • Posts: 326
Reply 3 on: Yesterday
Excellent

 

Did you know?

Oliver Wendell Holmes is credited with introducing the words "anesthesia" and "anesthetic" into the English language in 1846.

Did you know?

A headache when you wake up in the morning is indicative of sinusitis. Other symptoms of sinusitis can include fever, weakness, tiredness, a cough that may be more severe at night, and a runny nose or nasal congestion.

Did you know?

Drug-induced pharmacodynamic effects manifested in older adults include drug-induced renal toxicity, which can be a major factor when these adults are experiencing other kidney problems.

Did you know?

Thyroid conditions may make getting pregnant impossible.

Did you know?

Autoimmune diseases occur when the immune system destroys its own healthy tissues. When this occurs, white blood cells cannot distinguish between pathogens and normal cells.

For a complete list of videos, visit our video library