Hereditary anemia known as sickle cell disease (SCD) results from inheritance of a variant form of β-globin protein (βS), rather than the wild-type β-globin protein (βA). The βS protein does not migrate as far as the βA protein. Which of following does NOT explain why the gel electrophoresis lane containing the hemoglobin protein from the heterozygous (βAβS) individual has two protein bands?
◦ The band closer to the origin of migration contained βS protein and the band farther from the origin of migration contained βA protein.
◦ The βA protein has a higher electrophoretic mobility.
◦ The βS protein has a lower electrophoretic mobility.
◦ The protein bands migrated different distances based solely on differences in molecular weight.
◦ The different electrophoretic mobility of the two proteins was a result of differences in their molecular weight, charge, and/ or shape.
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Sickle Cell Anemia and Malaria