Question 1
What role do chromatin remodelers play in eukaryotic gene expression?
Question 2
A gene,
VTE4, from the sunflower of known sequence and well-understood transcriptional regulation, is available from another lab. The product of this gene enables sunflowers to to produce a significantly increased amount of vitamin E from a substrate produced in all plants. The plant
Brassica napus is harvested for canola oil. To produce canola oil with increased vitamin E, how would you proceed?
◦ Select
Brassica plants with the highest titer of the vitamin, isolate the genes responsible, and cause these genes to duplicate.
◦ Use a yeast system to engineer yeasts that produce the vitamin, and get manufacturers to add this to the canola oil during bottling.
◦ Introduce the gene and necessary cis elements into
Brassica, along with a reporter gene, then measure the vitamin E produced.
◦ Clone
VTE4 in
A. tumefaciens and use these bacteria to produce transgenic
Brassica.◦ Introduce
VTE4 into tomato plants or another food that humans eat more than
Brassica.
Transposon mutagenesis can be used to map a mutation in the genome sequence
Pre–Main-Sequence Evolutionary Tracks
Sequence of events from DNA to protein.
Sequence Series Arithmatic Progression Problem
An insertion sequence (IS), shown in purple
Mutations in the MAOA gene cosegregate with aggressive behavior