Question 1
What role do chromatin remodelers play in eukaryotic gene expression?
Question 2
A gene,
VTE4, from the sunflower of known sequence and well-understood transcriptional regulation, is available from another lab. The product of this gene enables sunflowers to to produce a significantly increased amount of vitamin E from a substrate produced in all plants. The plant
Brassica napus is harvested for canola oil. To produce canola oil with increased vitamin E, how would you proceed?
◦ Select
Brassica plants with the highest titer of the vitamin, isolate the genes responsible, and cause these genes to duplicate.
◦ Use a yeast system to engineer yeasts that produce the vitamin, and get manufacturers to add this to the canola oil during bottling.
◦ Introduce the gene and necessary cis elements into
Brassica, along with a reporter gene, then measure the vitamin E produced.
◦ Clone
VTE4 in
A. tumefaciens and use these bacteria to produce transgenic
Brassica.◦ Introduce
VTE4 into tomato plants or another food that humans eat more than
Brassica.
Step 3 of gene cloning: The cloning of a recombinant vector.
Gene duplication and the evolution of homologous genes
A microsatellite locus can show linkage to a disease gene
Common restriction enzymes, with their recognition sequence, DNA cutting patterns, and sources
An insertion sequence (IS), shown in purple
Comparisons of gene and genome organization