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Author Question: A dark pigmented colony is isolated from the leg wound of a fisherman from Florida. The ... (Read 516 times)

Caiter2013

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on: Jun 25, 2018
A dark pigmented colony is isolated from the leg wound of a fisherman from Florida. The gram-negative rod grows on sheep blood and MacConkey agars. The organism provides the following reactions:
 
  TSI: K/A Indole: no color change Lysine: no color change
   1 NaCL: no growth Arginine: purple Ornithine: no color change
   DNase: pink halo around the area of organism inoculum
  The microbiologist cannot read the oxidase reaction due to the dark pigment. What can he do to help achieve a valid oxidase reaction?
  A) Grow the organism under anaerobic conditions and retest
  B) Grow the organism on a non-nutrient agar and retest
  C) The oxidase test is not necessary for the identification of this organism
  D) Perform a modified oxidase test

Question 2

A microbiologist sets up a set of decarboxylase tubes for identification of a glucose fermenting, gram-negative rod.
 
  After 24 hours, the tubes reveal
  Base control: gray
  Arginine: purple
  Lysine: yellow
  Ornithine: purple
 
  These tubes should be interpreted as
  A) all positive. B) positive for arginine and ornithine.
  C) invalid. D) positive for lysine.


kaykay69

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Reply #1 on: Jun 25, 2018
Answer to Question 1

A

Answer to Question 2

C


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Caiter2013

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Reply #2 on: Jun 25, 2018
Correct answers!


kaykay69

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Reply #3 on: Jun 25, 2018
Great! Please up vote :D


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