A dark pigmented colony is isolated from the leg wound of a fisherman from Florida. The gram-negative rod grows on sheep blood and MacConkey agars. The organism provides the following reactions:
TSI: K/A Indole: no color change Lysine: no color change
1 NaCL: no growth Arginine: purple Ornithine: no color change
DNase: pink halo around the area of organism inoculum
The microbiologist cannot read the oxidase reaction due to the dark pigment. What can he do to help achieve a valid oxidase reaction?
A) Grow the organism under anaerobic conditions and retest
B) Grow the organism on a non-nutrient agar and retest
C) The oxidase test is not necessary for the identification of this organism
D) Perform a modified oxidase test
Question 2
A microbiologist sets up a set of decarboxylase tubes for identification of a glucose fermenting, gram-negative rod.
After 24 hours, the tubes reveal
Base control: gray
Arginine: purple
Lysine: yellow
Ornithine: purple
These tubes should be interpreted as
A) all positive. B) positive for arginine and ornithine.
C) invalid. D) positive for lysine.
Anoles mating, Gainesville, Florida
Pneumothorax (sucking chest wound).
Pigmented bands in nail of patient with dark skin
Swab the inside of the wound.
How to obtain more bacteria culture from already developed colony in HS medium
Pulegone, a cyclic monoterpene, can be isolated from the essential oil of pennyroyal, a member ...