This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Describe the steps involved in cytogenetic harvesting and banding, and explain the purpose of each. ... (Read 17 times)

Pineapplelove6

  • Hero Member
  • *****
  • Posts: 560
on: Jun 25, 2018
Describe the steps involved in cytogenetic harvesting and banding, and explain the purpose of each.
 
  What will be an ideal response?

Question 2

A patient is receiving treatment for Hodgkin lymphoma. Six weeks after cessation of the treatment, a cytogenetic analysis is performed. A structural aberration is found. Name the most probable aberration(s), and explain how these may occur.
 
  What will be an ideal response?


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by a Subject Expert

IAPPLET

  • Sr. Member
  • ****
  • Posts: 333
Reply #1 on: Jun 25, 2018
Answer to Question 1

Answer:
 Step 1: Arrest mitotically active cells in metaphase by incubating the sample with colchicine or colcemid that disrupts the spindle apparatus
 Step 2: Incubate the mixture in a hypotonic solution (0.075M KCl) to lyse the erythrocytes and swell the nucleated cells
 Step 3: Fix cells with Carnoy's fixative (3:1 mixture of methanol to glacial acetic acid)
 Step 4: Make a trial slide by adding 3-4 drops of the final cell suspension mixture onto a clean glass slide
 Step 5: Dry the trial slide and examine it by phase microscopy. If the first slide does not show optimal quality, the suspension can be concentrated or diluted to improve the slide's quality.
 Step 6: Aging of the slide: Heat the slide in a 60 C oven overnight to prepare for banding.
 Step 7: Banding: stain chromosomes; Q-banding is done with quinacrine; G-banding is done with Giemsa stain

Answer to Question 2

Answer: The most probable chromosomal aberrations are deletions of chromosomes 5 and 7. Also, 11q23 and 21q22 aberrations could be found in patients treated with chemotherapy. Aberrations in chromosomes 5 and 7 are related to treatment with alkylating agents. Aberrations in 11q23 and 21q22 are related to treatment with topoisomerase-II inhibitors.


Pineapplelove6

  • Member
  • Posts: 560
Reply 2 on: Jun 25, 2018
Gracias!

fatboyy09

  • Member
  • Posts: 358
Reply 3 on: Yesterday
Wow, this really help

« previous next »
 

Did you know?

The first documented use of surgical anesthesia in the United States was in Connecticut in 1844.

Did you know?

Over time, chronic hepatitis B virus and hepatitis C virus infections can progress to advanced liver disease, liver failure, and hepatocellular carcinoma. Unlike other forms, more than 80% of hepatitis C infections become chronic and lead to liver disease. When combined with hepatitis B, hepatitis C now accounts for 75% percent of all cases of liver disease around the world. Liver failure caused by hepatitis C is now leading cause of liver transplants in the United States.

Did you know?

If all the neurons in the human body were lined up, they would stretch more than 600 miles.

Did you know?

Hyperthyroidism leads to an increased rate of metabolism and affects about 1% of women but only 0.1% of men. For most people, this increased metabolic rate causes the thyroid gland to become enlarged (known as a goiter).

Did you know?

There are approximately 3 million unintended pregnancies in the United States each year.

Reversible amino acid modifications involved in regulation of protein activity Reversible amino acid modifications involved in regulation of protein activity
Steps in determining the structure of sperm whale myoglobin by x-ray crystallography Steps in determining the structure of sperm whale myoglobin by x-ray crystallography
Pathways involved in circadian rhythms Pathways involved in circadian rhythms
Surface molecules involved in the interaction between an antigen-presenting cell and a CD4+ T cell. Surface molecules involved in the interaction between an antigen-presenting cell and a CD4+ T cell.
Steps of the Heterogeneous immunoassays Steps of the Heterogeneous immunoassays
Describe the functions of fat in the body Describe the functions of fat in the body
Video: First Stage of Labor and Delivery Video

For a complete list of videos, visit our video library