Author Question: CD34 enumeration is useful in bone marrow and peripheral blood stem cell transplantation. For this ... (Read 111 times)

ts19998 · **on:** Jun 25, 2018

CD34 enumeration is useful in bone marrow and peripheral blood stem cell transplantation. For this enumeration, the EWGCCA recommendations include proper technique and interpretation of the flow cytometry analysis.

Which of the following standards was recommended for proper acquisition of events?

a. Include platelets and unlysed red blood cells and debris
b. Include only CD34 dim staining populations
c. Use bright fluorochrome conjugates of class II or III monoclonal antibodies that detect all glycoforms of CD34
d. Acquire only 10 CD34 positive cells

Question 2

Name at least two pitfalls in immunophenotyping mature lymphoid malignancies, and explain how you would correct this.

What will be an ideal response?

jamesnevil303 · **Reply #1 on:** Jun 25, 2018

Answer to Question 1

Correct Answer: C

Answer to Question 2

Answer: Some malignant B- cell lymphoproliferative disorders lack surface immunoglobulin and can be overlooked. Some lack surface antigens or an abnormal phenotype. Hodgkin lymphoma not only lacks many lymphoid cell antigens but also the neoplastic cells are rare, and a single cell suspension could be difficult to prepare because of fibrosis. Some abnormal phenotypes are not specific for a particular lymphoid neoplasm. It is important to use morphology together with immunophenotyping to help overcome these pitfalls.