Answer to Question 1
The DRI committee reviewed the available research on amino acids, but with next to no safety research in existence, the committee was unable to set Tolerable Upper Intake Levels for supplemental doses. Until research becomes available, no level of amino acid supplementation can be assumed to be safe for all people. A known side effect of these products is digestive disturbances: amino acids in concentrated supplements cause excess water to flow into the digestive tract, causing diarrhea. Anyone considering taking amino acid supplements should be cautious not to exceed levels normally found in foods.
The body is designed to handle whole proteins best. It breaks them into manageable pieces (dipeptides and tripeptides) and then splits these, a few at a time, simultaneously releasing them into the blood. This slow bit-by-bit assimilation is ideal because groups of chemically similar amino acids compete for the carriers that absorb them into the blood. An excess of one amino acid can produce such a demand for a carrier that it limits the absorption of another amino acid, creating a temporary imbalance.
Answer to Question 2
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Establishing a steady-state resting membrane potential.
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