This topic contains a solution. Click here to go to the answer

Author Question: What sequence of bases on the second strand of DNA is complementary to the following? (5') ... (Read 179 times)

ss2343

  • Hero Member
  • *****
  • Posts: 548

What sequence of bases on the second strand of DNA is complementary to the following?
 
   (5') GCATTA (3')



Question 2

In some instances, base pairs other than the familiar GC and AT matches can form. For these alternate pairs to form, one of the two bases must tautomerize. Based on this information, draw a CA pair and determine the number of hydrogen bonds that would stabilize the pair.

Question 3

Describe, in general terms, the steps involved in the automated synthesis of the DNA sequence GCT.



Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by a Subject Expert

akudia

  • Sr. Member
  • ****
  • Posts: 347
Answer to Question 1

(3') CGTAAT (5') or (5') TAATGC (3')

Answer to Question 2

Two hydrogen bonds can form in a CA pair.



Answer to Question 3

Step 1
Attachment of 5'-DMT-N-benzoyl-2'-deoxyaguanosine to the polymer support by a 3' ester linkage.
Step 2
Deprotection of the 5'-hydroxyl by cleavage of the DMT ether with dichloroacetic acid in CH2CL2.
Step 3
Phosphite formation between the free 5'-hydroxyl of the polymer-bound N-protected 2'-deoxyguanosine and the phosphoramidite protected 5'-DMT-2'-deoxycytidine in the presence of tetrazole followed by oxidation to the phosphate triester with I2.
Step 4
Repeat step 2 to deprotect the 5'-hydroxyl of the polymer-GC.
Step 5
Repeat step 3 to form the phosphate triester between 5'-hydroxyl of 2'-deoxycytidine residue and phosphoramidite protected 5'-DMT-N-benzoyl-2'-deoxythymidine.
Step 6
Cleave all protecting groups from the heterocyclic bases from the phosphates, as well as cleave the ester bond holding the DNA to the silica by treatment with aqueous ammonia.




ss2343

  • Member
  • Posts: 548
Reply 2 on: Aug 23, 2018
YES! Correct, THANKS for helping me on my review


Dnite

  • Member
  • Posts: 297
Reply 3 on: Yesterday
:D TYSM

 

Did you know?

Automated pill dispensing systems have alarms to alert patients when the correct dosing time has arrived. Most systems work with many varieties of medications, so patients who are taking a variety of drugs can still be in control of their dose regimen.

Did you know?

Children of people with alcoholism are more inclined to drink alcohol or use hard drugs. In fact, they are 400 times more likely to use hard drugs than those who do not have a family history of alcohol addiction.

Did you know?

It is important to read food labels and choose foods with low cholesterol and saturated trans fat. You should limit saturated fat to no higher than 6% of daily calories.

Did you know?

The use of salicylates dates back 2,500 years to Hippocrates's recommendation of willow bark (from which a salicylate is derived) as an aid to the pains of childbirth. However, overdosage of salicylates can harm body fluids, electrolytes, the CNS, the GI tract, the ears, the lungs, the blood, the liver, and the kidneys and cause coma or death.

Did you know?

Not getting enough sleep can greatly weaken the immune system. Lack of sleep makes you more likely to catch a cold, or more difficult to fight off an infection.

For a complete list of videos, visit our video library