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Author Question: What is the function of the high temperature step in PCR (polymerase chain reaction)? (Read 201 times)

robinn137

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on: Oct 17, 2019

Question 1

If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure, what would be the result?
◦ DNA replication would not occur at all.
◦ Many mistakes would occur.
◦ DNA replication would stop after one cycle.
◦ DNA replication would occur more slowly than normal.
◦ DNA replication would occur twice as fast as normal.

Question 2

What is the function of the high temperature step in PCR (polymerase chain reaction)?
◦ facilitate DNA synthesis
◦ allowing primers to bind complementary sequences
◦ activation of the DNA polymerase
◦ break hydrogen bonds for strand separation
◦ deactivate DNA polymerase to pause synthesis
Microbiology with Diseases by Body System ¦ Bauman ¦ 5th Edition
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Marked as best answer by robinn137 on Oct 17, 2019

yahahah24021

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Reply #1 on: Oct 17, 2019
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robinn137

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Reply 2 on: Oct 17, 2019
Great answer, keep it coming :)

covalentbond

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Reply 3 on: Yesterday
Thanks for the timely response, appreciate it

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