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Author Question: What is the function of the high temperature step in PCR (polymerase chain reaction)? (Read 200 times)

robinn137

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on: Oct 17, 2019

Question 1

If a researcher used Escherichia coli DNA polymerase instead of Thermus aquaticus DNA polymerase in the PCR procedure, what would be the result?
◦ DNA replication would not occur at all.
◦ Many mistakes would occur.
◦ DNA replication would stop after one cycle.
◦ DNA replication would occur more slowly than normal.
◦ DNA replication would occur twice as fast as normal.

Question 2

What is the function of the high temperature step in PCR (polymerase chain reaction)?
◦ facilitate DNA synthesis
◦ allowing primers to bind complementary sequences
◦ activation of the DNA polymerase
◦ break hydrogen bonds for strand separation
◦ deactivate DNA polymerase to pause synthesis
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Marked as best answer by robinn137 on Oct 17, 2019

yahahah24021

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Reply #1 on: Oct 17, 2019
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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robinn137

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Reply 2 on: Oct 17, 2019
YES! Correct, THANKS for helping me on my review

bbburns21

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Reply 3 on: Yesterday
Thanks for the timely response, appreciate it

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