If Single-Stranded Binding protein (SSB) is not present during DNA replication, what would you expect to see?
◦ The replisome complex would not assemble on the oriC region.
◦ The DNA cannot bend, so hydrogen bonds in the 13-mer region of oriC remain intact.
◦ Helicase activity is inhibited, so DNA strands cannot be separated.
◦ SSB carries the helicase protein to the open region of DNA, so hydrolysis and strand separation will not occur.
◦ SSB prevents reannealing of the separated strands, so strands would quickly reanneal and DNA replication cannot proceed.