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Author Question: A researcher is interested in testing the role for a highly-conserved amino acid in the function of ... (Read 55 times)

rosent76

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A researcher is interested in testing the role for a highly-conserved amino acid in the function of a mouse protein.  She has been unable to locate any pre-existing variants in this codon among a large number of available mouse strains. What might be her approach to generating such mutants?
◦ Use RNAi to knockdown the expression of the target gene.
◦ Use transposons to generate insertion alleles in the gene.
◦ Measure the expression of this gene in mice as they develop.
◦ Use CRISPR-cas to cause DNA cleavage at the codon encoding this amino acid.
◦ Use RNAi to interfere with translation of the gene.


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Marked as best answer by rosent76 on Jan 12, 2022

randomguy133

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rosent76

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Reply 2 on: Jan 12, 2022
Great answer, keep it coming :)


ashely1112

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Reply 3 on: Yesterday
Wow, this really help

 

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