Author Question: Suppose you have engineered a plasmid vector in order to produce a functional animal protein inE. ... (Read 73 times)

Mimi · **on:** Jan 12, 2022

Suppose you have engineered a plasmid vector in order to produce a functional animal protein in E. coli. But the product you collect, while it has the correct amino acid sequence, is in fact nonfunctional. What might you try that would have a greater chance of success?
◦ a eukaryotic expression vector that has sequences for regulating transcription
◦ using a host other than E. coli that does not have the same codon bias
◦ a eukaryotic expression vector that includes sequences for controlling posttranslational effects
◦ treatment of the posttranslation proteins to add signal sequences
◦ using yeast artificial chromosomes that can hold longer sequences

ankilker · **Reply #1 on:** Jan 12, 2022

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