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Author Question: Suppose you have engineered a plasmid vector in order to produce a functional animal protein inE. ... (Read 67 times)

Mimi

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on: Jan 12, 2022
Suppose you have engineered a plasmid vector in order to produce a functional animal protein in E. coli. But the product you collect, while it has the correct amino acid sequence, is in fact nonfunctional. What might you try that would have a greater chance of success?
◦ a eukaryotic expression vector that has sequences for regulating transcription
◦ using a host other than E. coli that does not have the same codon bias
◦ a eukaryotic expression vector that includes sequences for controlling posttranslational effects
◦ treatment of the posttranslation proteins to add signal sequences
◦ using yeast artificial chromosomes that can hold longer sequences
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Marked as best answer by Mimi on Jan 12, 2022

ankilker

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Reply #1 on: Jan 12, 2022
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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Mimi

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Reply 2 on: Jan 12, 2022
Thanks for the timely response, appreciate it

Dinolord

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Reply 3 on: Yesterday
Great answer, keep it coming :)

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