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Author Question: You have a linear DNA fragment of 1600 bp. To characterize it, you decide to make a restriction map. ... (Read 54 times)

Kthamas

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Question 1

Why might the expression of a transgene be especially abnormal in vertebrates with larger average size of genes and amount of heterochromatin in their genomes?
◦ position effect
◦ homologous recombination
◦ vector insufficiency
◦ mitotic recombination
◦ unequal crossing over

Question 2

You have a linear DNA fragment of 1600 bp. To characterize it, you decide to make a restriction map. Cutting with one enzyme, you show by electrophoresis that the fragments produced are 400 and 1200 bp. Cutting the same DNA with another enzyme you recover 700 and 900 bp fragments. After treatment with both enzymes, fragments were 300, 400, and 900 bp. Which of the following order of fragments, if any, best represents the restriction map?
◦ 900/ 400/ 300
◦ 400/ 900/ 300
◦ 300/ 400/ 900
◦ 400/ 300/ 900
◦ It is not possible to determine the order.


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Marked as best answer by Kthamas on Jan 12, 2022

FergA

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Kthamas

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Reply 2 on: Jan 12, 2022
Wow, this really help


Zebsrer

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Reply 3 on: Yesterday
Great answer, keep it coming :)

 

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