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Author Question: You have a linear DNA fragment of 1600 bp. To characterize it, you decide to make a restriction map. ... (Read 14 times)

Kthamas

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on: Jan 12, 2022

Question 1

Why might the expression of a transgene be especially abnormal in vertebrates with larger average size of genes and amount of heterochromatin in their genomes?
◦ position effect
◦ homologous recombination
◦ vector insufficiency
◦ mitotic recombination
◦ unequal crossing over

Question 2

You have a linear DNA fragment of 1600 bp. To characterize it, you decide to make a restriction map. Cutting with one enzyme, you show by electrophoresis that the fragments produced are 400 and 1200 bp. Cutting the same DNA with another enzyme you recover 700 and 900 bp fragments. After treatment with both enzymes, fragments were 300, 400, and 900 bp. Which of the following order of fragments, if any, best represents the restriction map?
◦ 900/ 400/ 300
◦ 400/ 900/ 300
◦ 300/ 400/ 900
◦ 400/ 300/ 900
◦ It is not possible to determine the order.
Genetic Analysis: An Integrated Approach ¦ Sanders, Bowman ¦ 3rd Edition
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Marked as best answer by Kthamas on Jan 12, 2022

FergA

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Reply #1 on: Jan 12, 2022
Lorsum iprem. Lorsus sur ipci. Lorsem sur iprem. Lorsum sur ipdi, lorsem sur ipci. Lorsum sur iprium, valum sur ipci et, vala sur ipci. Lorsem sur ipci, lorsa sur iprem. Valus sur ipdi. Lorsus sur iprium nunc, valem sur iprium. Valem sur ipdi. Lorsa sur iprium. Lorsum sur iprium. Valem sur ipdi. Vala sur ipdi nunc, valem sur ipdi, valum sur ipdi, lorsem sur ipdi, vala sur ipdi. Valem sur iprem nunc, lorsa sur iprium. Valum sur ipdi et, lorsus sur ipci. Valem sur iprem. Valem sur ipci. Lorsa sur iprium. Lorsem sur ipci, valus sur iprem. Lorsem sur iprem nunc, valus sur iprium.
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Kthamas

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Reply 2 on: Jan 12, 2022
Wow, this really help

LVPMS

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Reply 3 on: Yesterday
Great answer, keep it coming :)

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