A researcher is annotating a newly-sequenced chromosome that they constructed from contigs sequenced from a sample of Antarctic ice. The cell/organism that the chromosome came from is not known, however it yielded a single circular chromosome. You are asked to run a BLAST search to help this researcher identify the most related organism in GenBank. What would be the best strategy?
◦ Compare the most unusual portion of this newly-discovered chromosome to al known animal genomes.
◦ Perform many careful one-by-one comparisons between the newly-discovered DNA sequence and species selected from GenBank, one at a time.
◦ Use several ~1000bp portions of the DNA sequence to search for the closest matches from among the entire available database.
◦ Look for a nearly identical match to a very small part (20-40 bp) of the chromosome from all known ice bacteria.
◦ Look for an exact match to the whole chromosome.
Opener A researcher examines an agarose gel
Plot showing the number of genes on each human chromosome
A human fragile X chromosome
Meiosis Halves the Chromosome Number
Chromosome terminal deletion
Examples of Sequenced Genomes