A researcher has used in vitro mutagenesis to mutate a cloned gene and then has reinserted this into a cell. In order to have the mutated sequence disable the function of the gene, what must then occur?
A) recombination resulting in replacement of the wild type with the mutated gene
B) use of a microarray to verify continued expression of the original gene
C) replication of the cloned gene using a bacterial plasmid
D) transcription of the cloned gene using a BAC
E) attachment of the mutated gene to an existing mRNA to be translated
Step 2 of gene cloning: The actions of a restriction enzyme and DNA ligase to produce a recombinant
Transcription of a gene (Part 1)
Allele-specific oligonucleotide (ASO) testing for the -globin gene and sickle-cell anemia
Microarray procedure for analyzing gene expression in normal and cancer cells
Bone formation in normal mice and mutants for the runt gene Cbfa1
Gene Expression Control in a Fly