A researcher has used in vitro mutagenesis to mutate a cloned gene and then has reinserted this into a cell. In order to have the mutated sequence disable the function of the gene, what must then occur?
A) recombination resulting in replacement of the wild type with the mutated gene
B) use of a microarray to verify continued expression of the original gene
C) replication of the cloned gene using a bacterial plasmid
D) transcription of the cloned gene using a BAC
E) attachment of the mutated gene to an existing mRNA to be translated
The formation of a chimeric gene that is found in people with certain forms of leukemia.
In vitro oligonucleotide site-directed mutagenesis
Mechanisms of mutagenesis by nucleotide analogs
Test of Yanofsky’s Proposed trp Operon Gene Order
A test of Yanofsky’s proposed trp operon gene map
Transcription repression of the yeast GAL1 gene