A researcher has used in vitro mutagenesis to mutate a cloned gene and then has reinserted this into a cell. In order to have the mutated sequence disable the function of the gene, what must then occur?
A) recombination resulting in replacement of the wild type with the mutated gene
B) use of a microarray to verify continued expression of the original gene
C) replication of the cloned gene using a bacterial plasmid
D) transcription of the cloned gene using a BAC
E) attachment of the mutated gene to an existing mRNA to be translated
Kranz and Lörz first achieved in vitro plant fertilization
Gene Expression
A multistep model for colon cancer. The first mutation, in the APC gene, leads to the formation of p
Predicted polypeptide sequences translated from potential ORFs in the human TUBA3C gene
Mutations in the MAOA gene cosegregate with aggressive behavior
Allele-specific oligonucleotide (ASO) testing for the -globin gene and sickle-cell anemia