A researcher has used in vitro mutagenesis to mutate a cloned gene and then has reinserted this into a cell. In order to have the mutated sequence disable the function of the gene, what must then occur?
A) recombination resulting in replacement of the wild type with the mutated gene
B) use of a microarray to verify continued expression of the original gene
C) replication of the cloned gene using a bacterial plasmid
D) transcription of the cloned gene using a BAC
E) attachment of the mutated gene to an existing mRNA to be translated
A missense mutation that converts the normal ras gene into an oncogene.
Gene silencing influences flower colour in petunia.
Gene duplication and mutation: one path to generate new enzymatic activities
Mechanisms of gene regulation by RNA-induced gene silencing
Assembly of a к light-chain gene in a B cell
Gene content of mitochondrial genomes