A researcher has used in vitro mutagenesis to mutate a cloned gene and then has reinserted this into a cell. In order to have the mutated sequence disable the function of the gene, what must then occur?
A) recombination resulting in replacement of the wild type with the mutated gene
B) use of a microarray to verify continued expression of the original gene
C) replication of the cloned gene using a bacterial plasmid
D) transcription of the cloned gene using a BAC
E) attachment of the mutated gene to an existing mRNA to be translated
Gene cloning
Relationship between Hox gene number and body complexity in different types of animals.
Galactose gene expression
Scheme of the principle of the fluorescent in situ hybridization to localize a gene in the nucleus.
Creation of recombinant DNA molecules in vitro
DNA sequence variation in parts of the Drosophila Adh gene