Identify Prussian blue iron stain results in the following ...

[Shelles]

Identify Prussian blue iron stain results in the following disorders and explain your answers:
 
  a. Iron-deficiency anemia
  b. Anemia of chronic disease
  c. Thalassemia
  d. Sideroblastic anemia
 
  What will be an ideal response?

Question 2

Name disorders in which the following should be included in the workup of a hematologic disorder. Explain why they are used.
 
  a. Flow cytometry
  b. Cytogenetics
  c. Molecular genetics
  d. Cytochemical staining
 
  What will be an ideal response?

[Chocorrol77]

Answer to Question 1

Answer:
Iron-deficiency anemia: decreased; the etiology of IDA is such that when a patient's iron need exceeds intake, the body utilizes its iron stores and decreases them; is often one of the first signs of iron-deficiency anemia
Anemia of chronic disease: adequate to increased; the problem is impaired ferrokinetics. There is adequate intake of iron but poor utilization of it. The primary protein abnormality that is responsible for this abnormal utilization is hepcidin. Hepcidin is produced in response to inflammatory cytokines, which are increased in patients with ACD, so the disorder often arises from an exacerbated inflammatory response.
Thalassemia: adequate to increased; the defect is improper globin chain synthesis, not iron usage.
Sideroblastic anemia: increased; this is the opposite of IDA. Iron intake exceeds the body's need for iron and can be improperly utilized.

Answer to Question 2

Answer:
Flow cytometry: malignant lymphomas and leukemias; evaluates for minimal residual disease after chemotherapy
Cytogenetics: acute leukemias, malignant lymphomas, MDS, and MPDs; helps determine diagnosis and prognosis. Also can detect relapse.
Molecular genetics: hematolymphoid malignancies; can detect minimal residual disease
Cytochemical staining: subsets of acute leukemia; differential diagnosis of acute and chronic leukemias, such as ALL, AML, CML, CLL, and HCL.

[Shelles]

TYSM

[kjohnson]

Excellent