This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Explain the role of the laboratory professional in stem cell transplantation. What will be an ... (Read 87 times)

mckennatimberlake

  • Hero Member
  • *****
  • Posts: 559
on: Jun 25, 2018
Explain the role of the laboratory professional in stem cell transplantation.
 
  What will be an ideal response?

Question 2

Explain the detection and significance of chimerisms in stem cell transplantation.
 
  What will be an ideal response?


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by a Subject Expert

yuyiding

  • Sr. Member
  • ****
  • Posts: 357
Reply #1 on: Jun 25, 2018
Answer to Question 1

Answer: The role of the laboratory professional is inherent in all phases of testing for stem cell transplant:
Pretransplant testing:
 HLA compatibility testing
 ABO compatibility testing (if needed)
 Absolute neutrophil count (CBC) for chemotherapy dosage
Peritransplant testing:
 Apheresis of stem cells
 WBC/MNC counts
 CD34 enumeration by flow cytometry
 Processing and cryopreservation of stem cells
 Bacterial, viral, fungal cultures
 Cell cultures for enumeration of CFUs
Post-transplant testing:
 Short-term engraftment  CBC
 Long-term engraftment  chimerism studies
 Investigation of possible complications

Answer to Question 2

The presence of chimerisms indicates complete infusion of donor HSCs into the recipient. The method of detection is through variable number tandem repeat (VNTR) polymorphisms, which separate out donor DNA/RNA from recipient DNA/RNA. Complete chimerisms exist if there is only donor DNA/RNA present. A complete chimerism indicates a successful stem cell transplant.


mckennatimberlake

  • Hero Member
  • *****
  • Posts: 559
Reply #2 on: Jun 25, 2018
Thank you :)


yuyiding

  • Sr. Member
  • ****
  • Posts: 357
Reply #3 on: Jun 25, 2018
Happy to help you


« previous next »
 

Did you know?

One way to reduce acid reflux is to lose two or three pounds. Most people lose weight in the belly area first when they increase exercise, meaning that heartburn can be reduced quickly by this method.

Did you know?

Fewer than 10% of babies are born on their exact due dates, 50% are born within 1 week of the due date, and 90% are born within 2 weeks of the date.

Did you know?

The average adult has about 21 square feet of skin.

Did you know?

To maintain good kidney function, you should drink at least 3 quarts of water daily. Water dilutes urine and helps prevent concentrations of salts and minerals that can lead to kidney stone formation. Chronic dehydration is a major contributor to the development of kidney stones.

Did you know?

A strange skin disease referred to as Morgellons has occurred in the southern United States and in California. Symptoms include slowly healing sores, joint pain, persistent fatigue, and a sensation of things crawling through the skin. Another symptom is strange-looking, threadlike extrusions coming out of the skin.

Response of a yeast cell to glucose Response of a yeast cell to glucose
General features of a growth factor or hormone signalling pathway that promotes cell division General features of a growth factor or hormone signalling pathway that promotes cell division
Stem Cell Hierarchy Stem Cell Hierarchy
The three stages we go through to develop the ability of taking the role of the other. The three stages we go through to develop the ability of taking the role of the other.
Each cell nucleus throughout the body contains the genes, DNA, and chromosomes that make up the ... Each cell nucleus throughout the body contains the genes, DNA, and chromosomes that make up the ...
Overview of Cell Organelles Overview of Cell Organelles
Video: Exercise and cardiac output

For a complete list of videos, visit our video library