This topic contains a solution. Click here to go to the answer
« previous next »

Author Question: Explain the role of the laboratory professional in stem cell transplantation. What will be an ... (Read 61 times)

mckennatimberlake

  • Hero Member
  • *****
  • Posts: 559
on: Jun 25, 2018
Explain the role of the laboratory professional in stem cell transplantation.
 
  What will be an ideal response?

Question 2

Explain the detection and significance of chimerisms in stem cell transplantation.
 
  What will be an ideal response?


Related Topics

Need homework help now?

Ask unlimited questions for free

Ask a Question
Marked as best answer by a Subject Expert

yuyiding

  • Sr. Member
  • ****
  • Posts: 357
Reply #1 on: Jun 25, 2018
Answer to Question 1

Answer: The role of the laboratory professional is inherent in all phases of testing for stem cell transplant:
Pretransplant testing:
 HLA compatibility testing
 ABO compatibility testing (if needed)
 Absolute neutrophil count (CBC) for chemotherapy dosage
Peritransplant testing:
 Apheresis of stem cells
 WBC/MNC counts
 CD34 enumeration by flow cytometry
 Processing and cryopreservation of stem cells
 Bacterial, viral, fungal cultures
 Cell cultures for enumeration of CFUs
Post-transplant testing:
 Short-term engraftment  CBC
 Long-term engraftment  chimerism studies
 Investigation of possible complications

Answer to Question 2

The presence of chimerisms indicates complete infusion of donor HSCs into the recipient. The method of detection is through variable number tandem repeat (VNTR) polymorphisms, which separate out donor DNA/RNA from recipient DNA/RNA. Complete chimerisms exist if there is only donor DNA/RNA present. A complete chimerism indicates a successful stem cell transplant.


mckennatimberlake

  • Hero Member
  • *****
  • Posts: 559
Reply #2 on: Jun 25, 2018
Thank you :)


yuyiding

  • Sr. Member
  • ****
  • Posts: 357
Reply #3 on: Jun 25, 2018
Happy to help you


« previous next »
 

Did you know?

More than 34,000 trademarked medication names and more than 10,000 generic medication names are in use in the United States.

Did you know?

Cucumber slices relieve headaches by tightening blood vessels, reducing blood flow to the area, and relieving pressure.

Did you know?

Egg cells are about the size of a grain of sand. They are formed inside of a female's ovaries before she is even born.

Did you know?

Prostaglandins were first isolated from human semen in Sweden in the 1930s. They were so named because the researcher thought that they came from the prostate gland. In fact, prostaglandins exist and are synthesized in almost every cell of the body.

Did you know?

Fewer than 10% of babies are born on their exact due dates, 50% are born within 1 week of the due date, and 90% are born within 2 weeks of the date.

Structure of cellulose, the main macromolecule of the primary cell wall Structure of cellulose, the main macromolecule of the primary cell wall
Neuron (nerve cell) structure. Neuron (nerve cell) structure.
Activity of a Hippocampal Grid Cell Activity of a Hippocampal Grid Cell
Animal Cell Animal Cell
Locations of major metabolic pathways within a eukaryotic cell Locations of major metabolic pathways within a eukaryotic cell
Outgroup role in relative rate test Outgroup role in relative rate test
Video: ETC Protein Complexes & Chemiosmosis (Total ATP Production and ATP Synthase)

For a complete list of videos, visit our video library