Question 1
Given the following table:
Supplement
where numbers 1 through 4 indicate four supplements that must be added to sustain growth of the organism, determine a possible metabolic pathway that would give the results seen for the four mutant strains, A through D.
Question 2
The following table presents the effect of different media on the growth response of tryptophan mutations in
Salmonella typhimurium (+ = growth, - = no growth).
Medium Supplemented with
| Strain | No Supplement | IGP | AA | IN | TRY |
(a) Construct the biochemical pathway for the compounds IGP, AA, IN, and TRY based on these data.
(b) Place strains of bacteria (mutations) in the appropriate steps in the pathway.
(c) In bacteria, it is often possible to make partial diploid strains. Assume that a diploid strain was made containing the complete genomes of the
trp-2 and
trp-1 strains. Would this diploid strain be able to grow on the unsupplemented medium? Explain your answer.
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