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Author Question: The following table presents the effect of different media on the growth response of tryptophan ... (Read 47 times)

gonzo233

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Question 1

Given the following table:

Supplement
1234
Strain A+-+-
Strain B+++-
Strain C++++
Strain D--+-

where numbers 1 through 4 indicate four supplements that must be added to sustain growth of the organism, determine a possible metabolic pathway that would give the results seen for the four mutant strains, A through D.

Question 2

The following table presents the effect of different media on the growth response of tryptophan mutations in Salmonella typhimurium (+ = growth, - = no growth).

Medium Supplemented with
StrainNo SupplementIGPAAINTRY
trp-8-++++
trp-2-+-++
trp-3---++
trp-1----+

(a) Construct the biochemical pathway for the compounds IGP, AA, IN, and TRY based on these data.
(b) Place strains of bacteria (mutations) in the appropriate steps in the pathway.
(c) In bacteria, it is often possible to make partial diploid strains. Assume that a diploid strain was made containing the complete genomes of the trp-2 and trp-1 strains. Would this diploid strain be able to grow on the unsupplemented medium? Explain your answer.


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Marked as best answer by gonzo233 on Mar 26, 2020

iceage

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gonzo233

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Reply 2 on: Mar 26, 2020
Excellent


amit

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Reply 3 on: Yesterday
Thanks for the timely response, appreciate it

 

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