Question 1
List two especially useful characteristics of cloning vectors.
◦ reverse transcriptase and ligase activities
◦ virulence and lysogenicity
◦ nonautonomous replication and transposition
◦ high copy number and antibiotic resistance gene(s)
◦ ability to integrate into the host chromosome and then causing a lytic cycle
Question 2
Vectors such as pUC18 and others of the pUC series contain a large number of restriction enzyme sites clustered in one region. Which term describes this advantageous arrangement of restriction sites?
◦ multiple cloning site
◦ complementation
◦ consensus sequence
◦ β-galactosidase
◦ palindrome